Contagem total de hemócitos de camarões marinhos Litopenaeus vannamei (Boone, 1931) cultivados no litoral Norte de Pernambuco

Abstract

The study of blood cells, also known as hematology, has become for aquaculture a valuable tool in understanding the physiological changes that occur in organisms, however the shrimp, there is still much to discover about if the hemolymph. Considering the above objective of this work: to evaluate the effectiveness of the anticoagulant sodium citrate (10%) in blood tests, to check the influence of cooling on the total count of hemocytes (CTH), haematological data correlate with the presence of Vibrio in the hemolymph, to identify the influence of age and seasonality in the total count of haemocytes in shrimp Litopenaeus vannamei. The species were caught shrimp Litopenaeus vannamei from two nurseries of land (A and B) of mariculture Santa Cruz, located in the city of Goiana-PE. The collections are made weekly, for two cycles of cultivation (dry season and rainy season). It is the examination fresh (macro and microscopic) in all animals sampled. The total counts of hemocytes (cells/mm3) were performed immediately after collection and after five hours of chilling in a Neubauer chamber. Immediately after the counting, the samples intended for bacteriological analysis were transported in temperature to the laboratory of health of aquatic animals (LASAq), Universidade Federal Rural de Pernambuco. The data concerning the influence of cooling on the hemocytes were analyzed using the techniques of mathematical modeling (P <0.05), however the data for the bacteriological analysis,examining changes in the fresh and CTH, the shrimps were divided into four groups: cameroon with injury and with bacteria (CLCB), with injury and without bacteria (CLSB) without injury and with bacteria (SLCB) and without injury and without bacteria (SLSB). The results were submitted to analysis of variance (ANOVA) and when significant, the Tukey test for comparison between means, is adopting a significance level of 5%. At the end of the research concluded that the anticoagulant sodium citrate only adequately preserves the hemolymph of the shrimp Litopenaeus vannamei to CTH performed immediately after collection, and the CTH is not associated with the presence of Vibrio spp and examination with lesions detected in the fresh and therefore not a good tool for immediate recognition of changes in the health of marine shrimp Litopenaeus vannamei.