Avaliação fitoquímica e da atividade antimicrobiana dos extratos hidroalcóólico e hexânico das folhas de Melaleuca leucadendra (Myrtaceae) em Staphylococcus aureus isolados de mastite bovina

Abstract

The objective of this study was to carry out the phytochemical evaluation and antimicrobial activity of the hydroalcoholic and hexanic extract of the leaves of Melaleuca leucadendra (Myrtaceae), at a concentration of 20mg/mL, on Staphylococcus aureus isolated from bovine mastitis. After plant identification, extracts were prepared and subjected to phytochemical and antibacterial tests. Verification of the presence of metabolite classes was carried out by chromatographic analysis using traditional techniques and verification of compounds by mass spectrometry coupled to electrosplay HPLC-DAD-ESI(+)-MS/MS (micrOTOF-QII) (ESI-MS) ; the hydroalcoholic and hexane extract were analyzed by gas chromatography-mass spectrometry (GC-ME). To evaluate the antibacterial action, the inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were verified in isolates of Staphylococcus aureus from bovine mastitis. The results verified the presence of terpenes, glycosylated flavonoids, aglycone flavonoids, triterpenes, tannin and spectrometry identified 13 chemical superclasses with 88 compounds. In the GC-MS chromatogram, 37 peaks were found, corresponding to 37 chemical compounds. The hydroalcoholic and hexane extracts presented metabolites with biological activity proven in the specialized literature. In turn, the same extracts showed antimicrobial activity against strains of Staphylococcus aureus that cause bovine mastitis, proving to be an alternative in the treatment of the disease.