Obtenção de proteases por particionamento dos resíduos de tainha (Mugil liza) visando sua aplicação biotecnológica

Abstract

By-products from the fishing industry are rich in biological compounds, such as proteases, and their recovery has technical advantages over chemical ones and has wide industrial applications, mainly in medicines, cosmetics, food, textiles and animal feed, due to its property of high activity over a wide pH range and at different temperatures. Several techniques for recovery, identification and purification of enzymes capable of cleaving the peptide bonds of collagen molecules and subsequently degrading them into small peptides from these residues have been used, such as the semipurification technique by aqueous two-phase system (SDFA) which was applied to neglected mullet (Mugil liza) waste (viscera) for the treatment of enzymes with collagenolytic activity. The crude extract (EB) obtained from the intestinal viscera of Mullet (Mugil liza) showed the following activities: total protein 1.02 U/mg; collagenolytic activity: 152.0 U/mg volumetric; 161 U/mg specific collagenolytic activity; 7.35 U/mL of proteolytic/proteasic activity; and, finally, 14.2 mg/mL of specific proteolytic activity. In the two-phase PEG/citrate aqueous system, according to the 2³ factorial design, using the variables Partition coefficient (K), Yield (Y), and Purification factor (FP), it was possible to observe that the extraction yields (Y ) obtained ranged from 89 to 124.34% and from 242.11 to 435.61% for protease and proteases with collagenolytic activity, respectively. The results showed the advantage of this semi-purification technique regarding the extraction and purification of collagenolytic enzymes with industrial application. The fish collagen peptides, highlighted in the integrative review, are important in the global collagen peptides market and are important in the development of biomaterials that constitute pharmaceutical, cosmetic and food products. , properties, preparation methods, and applications in different industries. It specifically encompasses collagen peptides applied in the pharmaceutical, cosmetics and food industries. In addition, it emphasizes the regulatory aspects of commercial collagen peptide by highlighting information related to certain ingredients. Also covering patents and existing commercial collagen peptide products with indications. This review provides information on collagen peptides with industrial applications covering essential aspects for future research in this specific field.