Isolamento de metabólitos secundários de Caulerpa racemosa e avaliação farmacológica em modelo de infecção por Leishmania sp

Abstract

As seaweed is a promising source of secondary metabolites that can be used in the pharmaceutical industry. Caulerpa racemosa is known as “sea grapes” and lives close to the sandy surface in deep waters. It is known for biological activities such as antibacterial, antifungal, antidiabetic, antioxidant, antinociceptive, anti-inflammatory and leishmanicidal action. The present work aimed to analyze the chemical profile and the leishmanicidal activity of the activated fractions through the continuous phase extraction (SPE) of the algae C. racemosa against a species of Leishmania amazonensis. An algae was extracted with ethanol and the ethanol extractor was subjected to a relationship in the SPE with methanol and water in different proportions, being considered six fractions that were analyzed by UPLC-ESI-qTOF-MSE and subjected to cell viability tests in L. amazonensis. An analysis selected to identify alkaloids: monomethyl caulerpinin, caulerpine, caulerpinic acid, caulersin, caulerpinic acid and a new compound derived from caulerpin which was used and identified by spectroscopic techniques such as methyl ethyl caulerpinato, a leishmanicidal activity against L amazonensis from ethanol extract and from fractions of C. racemosa were significantly affected at 5, 10 and 20 μg / mL. As the main substances identified in the species C. racemosa, alkaloids used in kaolin and both or crude ethanolic extract, such as the SPE fractions reported leishmanicidal activity were found.