Avaliação morfológica e morfométrica de feridas cutâneas em ratos wistar, tratadas com pomada composta por alecrim da chapada (Lippia gracilis Schauer) e aroeira (Schinus terebinthifolius Raddi)

Abstract

The objective of this work was to evaluate the healing activity of ointment composed of essential oils of rosemary (Lippia gracilis Schauer) and aroeira (Schinus terebenthifolius Raddi) in cutaneous wounds of rats. Wistar rats, adult males (n=40), were divided into two groups: Treated Group (GT) - wounds treated with ointment composed of essential oil of the leaves of L. gracilis Schauer and S. terebinthifolius Raddi at the concentration of 5% each , incorporated into Lanette base (n=20) and Control Group (GC) - wounds treated with ointment containing Lanette base alone (n=20). Each animal was submitted to two incisions in the skin, in the dorsolateral thoracic region, in the right and left antimeres. The wounds were measured on postoperative day 0, 3, 7, 14 and 21 (PO), with the aid of the digital caliper to evaluate the degree of contraction and analyzed macroscopically. Histological sections stained with Hematoxylin-Eosin (HE) and Trichrome of Gomori were used for morphological and morphometric evaluations by quantification of blood vessels and determination of density of collagen fibers. The data were submitted to the ANOVA test complemented by the Tukey-Kramer test (p<0.05). The phytochemical study revealed that the OE of the leaves of S. terebinthifolius Raddi has ρ-vinyl-Guaiacol and α-Terpineol in greater quantity and of L. gracilis Schauer presented Carvacrol and Thymol as major constituents. There was no statistical difference between groups in the analyzed data. The rosemary ointment of the veneer with aroeira, although presenting compounds important for healing, was not able to favor the cicatricial process in this experiment. However, we emphasize that further research is needed on the associated use of these two oils, as well as their action in the healing process.