Potencialidade do soro de queijo frente a outras fontes de carbono para produção de β-galactosidase por Kluyveromyces lactis URM 6684

Abstract

Cheese whey is the aqueous portion milk rich in lactose, which separates the curd during cheese manufacture which makes it of great importance effluent dairy industries. With the rapid advances in microbial biotechnology, it is considered good alternative to its use, as a substrate inexpensive and available in obtaining β-galactosidase, which is widely used for pharmaceutical and food industries. The sources most widely used for its production are yeasts, as the species Kluyveromyces lactis and Kluyveromyces marxianus. The objective of this study was to evaluate the feasibility of using the cheese whey, in isolation or in combination with other carbon sources for the production of β-galactosidase enzyme, using Kluyveromyces lactis URM 6684, using the planning simplex centroid mixture. 9 fermentations were carried out using three sources of carbon: cheese whey, lactose and sucrose PA PA. During the study it was determined biomass, pH and after cell disruption, enzymatic activity. With regard to biomass, medium with sucrose had better results E5 (7,8 g / L), E8 (6,8 g / L) and E6 (6,1 g / L). The enzyme activity is presented to best results in medium with cheese whey: E1 (0,65 U / ml), E4 (0,33 U / ml) and E6 (0,40 U / ml). Statistical analysis showed that the enzyme activity showed the best fit to the quadratic and cubic models, which presented the best fit quadratic and cubic models, with coefficients, RA2 = 0.86 and RA2 = 0.93. The results showed that whey was presented as a suitable source of carbon and low cost for production of the enzyme, adding more value to its use, and reduce the costs of production of β-galactosidase enzyme, increasing its availability commerce more affordable and enabling their application in the dairy food industry.