Cowpea severe mosaic virus : diagnóstico, estudo de herança e identificação de marcadores moleculares associados à resistência

Abstract

The cowpea, it plays important economical-social in the agriculture of the areas North and Northeast of Brazil. The Northeast is the largest Brazilian producer, where it is used broadly in the human feeding to the detriment of the common bean. Approximately 60% of thetotal area of bean in the Northeast are cultivated with cowpea. The culture generates 2,4 million direct jobs and it supplies the table of 27,5 million Northeasterners, what accredits theculture to receive support of the fomentation organs to the research.In the Brazilian Northeast, the mosaic,provoked by virus, due to the frequency and severity with that happen, they blunt as the most important diseases, being constituted in factor limitiong of the production. Among the viral illnesses, the severe mosaic of the cowpea, caused by Cowpea severe mosaic virus (CPSMV) it is responsible for losses of the order 60% to 100%. This way, the present study had for objectives determine the inheritance of the resistance and identify associated markers with resistance. The individuals' selection F2 to CPSMV was accomplished through inoculation with extract vegetable and the behavior of the population of the cowpea for observation symptoms and qui-square test. The selection of primers polymorphic was accomplished by the analysis of bulkers segregant (BSA). Primers potentially associated with resistance or susceptibility to CPSMV were evaluated in the population segregant. Of hte 85 primers tested, the primer VM 70 proved to be co-segregating with. This marker primers represents a new tool to assist in obtaining resistant cultivars, faster, accorate and economical in the analysis of the diagnosis of the virus of the severe mosaic it was verified that the technique of RT-PCR, comes as a fast and efficient method